Assay kit and antibody for human low molecular weight cd14

Details Assay kit and antibody for human low molecular weight cd14 Assay kit and antibody for human low molecular weight cd14

C - Chemistry, Metallurgy

07

K

C07K 16/28 (2006.01) C07K 7/00 (2006.01) C07K 14/705 (2006.01) C12N 15/06 (2006.01) C12P 21/08 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2506580

It is intended to provide an antibody constructed by using, as an antigen, a peptide comprising from 8 to 30 amino acid residues selected from among the amino acid residues at the 1- to 68-positions in the amino acid sequence of human high-molecular weight CD14; or an antibody binding to a peptide comprising a specific amino acid sequence in the 1- to 68-positions as described above. Using an assay kit for assaying human low-molecular weight CD14 (preferably a sandwich method) with the use of the above antibody, human low-molecular weight CD14 can be highly sensitively, conveniently and specifically assayed qualitatively or quantitatively, which is useful in diagnosing patients with sepsis.

L'invention concerne un anticorps construit au moyen d'un peptide, en tant qu'antigène, comprenant 8 à 10 résidus d'acides aminés choisis dans les résidus d'acides aminés aux positions 1 à 68 dans la séquence d'acide aminé de CD14 humain de poids moléculaire élevé ; ou un anticorps se liant à un peptide comprenant une séquence d'acide aminé spécifique aux positions 1 à 68 comme décrit ci-dessus. L'utilisation d'une trousse d'analyse pour analyser le CD14 humain de faible poids moléculaire (de préférence selon un procédé en sandwich), avec utilisation de l'anticorps ci-dessus, le CD14 humain de faible poids moléculaire peut être analysé de manière hautement sensible, appropriée et spécifique qualitativement ou quantitativement, ce qui est utile lors du diagnostic de patients atteints de sepsie.

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