Assay kit for camp and/or cgmp and method of using the same

G - Physics – 01 – N

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G01N 23/00 (2006.01) G01N 33/53 (2006.01) G01N 33/537 (2006.01)

Patent

CA 1092022

ABSTRACT OF THE DISCLOSURE A cAMP and/or cGMP assay kit comprises, in combi- nation: a Regent 1 comprising succinyl cyclic AMP tyrosine methyl ester and/or succinyl cyclic GMP tyrosine methyl eater labeled with radioactive iodine; a Reagent 2 comprising an anti-cAMP serum and/or anti-cGMP serum; a Reagent 3 comprising an imidazole buffer solu- tion of 0.1 M or higher concentration; a Reagent 4 comprising a material for separation; a Reagent 5 comprising a solution in an organic solvent of succinic anhydride; a Reagent 6 comprising an organic tertiary amine; and a Reagent 7 comprising a standard solution of cAMP and/or cGMP. These reagents may be used in accordance with ordinary practice for radioimmunoassay of cAMP and cGMP.

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