C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/00 (2006.01) C12Q 1/16 (2006.01) G01N 33/532 (2006.01) G01N 33/543 (2006.01)
Patent
CA 2343728
Disclosed is a member comprising an interactive material which is covalently bonded to a support body by a linker material. The member can be used as a part of an assay and the support body may include a scintillator material. The figure shows a graph illustrating kinetic activity of various amounts of purified recombinant PLC .gamma.1 on [3H] PIP2 coated 96-well phospholipid FlashPlate~. Different concentrations of purified recombinant PLC were added to individual wells in 0.1 ml of 50 mM NaPi, pH 6.7, 0.1 M NaCl, 0.1 mM CaCl2, 0.1 mM EGTA and the release of radioactivity was monitored as a function of time on a Packard TopCount~.
L'invention concerne un élément comprenant une matière interactive, laquelle est liée par covalence à un corps support au moyen d'une substance de liaison. L'élément de cette invention peut être utilisé comme élément d'une analyse, ledit corps support pouvant renfermer une substance scintillante. La figure montre un graphique illustrant l'activité cinétique de plusieurs quantités d'enzymes PLC .gamma.1 purifiées recombinées sur une plaque Flashplate~ phospholipidique à 96 cupules revêtue de [?3¿H] PIP¿2?. Selon cette invention, on ajoute différentes concentrations d'enzymes PLC purifiées recombinées dans chaque cupule, avec 0,1 ml de NaPi à 50mM, un pH de 6,7, 0,1M de NaCl, 0,1 mM de Cacl2, et 0,1mM d'EGTA, puis on surveille la radioactivité libérée, en tant que fonction temporelle, sur un dispositif Packard TopCount~.
Bembenek Michael E.
Bobrow Mark N.
Mullinax Thomas R.
Nen Life Science Products Inc.
Ridout & Maybee Llp
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