Assay method and device

G - Physics – 01 – N

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167/37, 150/9

G01N 33/50 (2006.01) C12Q 1/00 (2006.01) G01N 23/06 (2006.01) G01N 33/558 (2006.01)

Patent

CA 1184114

ABSTRACT OF THE DISCLOSURE A method and device for assaying a test substance utilizing a primary absorbent substance for selectively allowing only a quantity of an analytical reagent proportional to the quantity of test substance to pass therethrough when test substance and analytical reagent are contacted with the primary absorbent. An analytical absorbent is disposed in a series of zones for sequen- tially absorbing the analytical reagent which passes through the primary absorbent so that detection of the last zone of absorbed analytical reagent indicates the quantity of test substance. The method comprises passing test substance and analytical reagent through the primary absorbent and then the analytical absorbent followed by detection of the last zone in which analytical reagent is absorbed. The device comprises a funnel with the primary absorbent therein for directing the test substance and analytical reagent to a narrow tube holding the analytical absorbent.

398198

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