C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/34 (2006.01) C12M 1/40 (2006.01) C12Q 1/54 (2006.01)
Patent
CA 2146207
The present invention provides an assay method for hemicellulases comprising a) dyeing, using a reactive dye, wood or other vegetable material that has been mechanically decomposed or homogenized; and b) adding an enzyme to a dyed product produced in step a); and c) after an incubation period of from 2 minutes to 4 hours, separating a liquid component from the dyed product, e.g. by a simple filtration, and determining the amount of dyestuff liberated in the the separated solution by spectrophotometric means. A combined mixing and dispensing device for use in the method of the present invention is also provided.
Fisk Spencer
Freiermuth Beat
Gaertner Alfred
Werthemann Dieter
Ag Ciba-Geigy
Fetherstonhaugh & Co.
Genencor International Inc.
Nalco Chemical Company
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