Assay method and probe for polynucleotide sequences

Details Assay method and probe for polynucleotide sequences Assay method and probe for polynucleotide sequences

C - Chemistry, Metallurgy

12

N

167/37, 195/1.22

C12N 15/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 1214410

ASSAY METHOD AND PROBE FOR POLYNUCLEOTIDE SEQUENCES Abstract of the Disclosure An asssay method for the detection of a specific nucleotide target sequence in a polynucleotide test extract is disclosed which utilizes a polynucleotide modified probe including both a cDNA sequence sub- stantially complementary to the specific target sequence and a protein binding sequence. The assay is conducted by exposing the modified probe to the polynucleotide test extract for hybridization and then exposing the complex to the protein which binds to the protein binding sequence. An assay, such as an immunossay, can then be conducted on the test sample to indicate the presence of the specific target sequence by detecting the presence of the binding protein.

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