Assay method for a substance with a specific sugar chain

Details Assay method for a substance with a specific sugar chain Assay method for a substance with a specific sugar chain

G - Physics

01

N

150/11, 167/37

G01N 33/53 (2006.01) G01N 33/574 (2006.01) G01N 33/66 (2006.01) G01N 33/68 (2006.01) G01N 33/92 (2006.01)

Patent

CA 2017342

ABSTRACT An assay method for a substance with a specific sugar chain, comprising; (A) adding a functional-group-bound antibody which recognizes a substance with a specific sugar chain to a sample solution containing the substance, followed by binding a complex comprising the substance and the antibody to a carrier via the functional group, (B) selectively dissociating the complex comprising the substance and the antibody from the carrier, (C) binding the substance and a lectin which recognizes the specific sugar chain thereof together, and (D) determining the amount of the complex comprising the lectin, the substance and the antibody is described. According to the present invention, a glycoprotein with a specific sugar chain can be assayed simply and with high sensitivity without any influence from the coexisting substances in the sample.

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