Assay method for analyte in living body

G - Physics – 01 – N

Patent

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G01N 33/553 (2006.01) G01N 33/537 (2006.01)

Patent

CA 2160607

A process for assaying a biological substance is provided. In the assay, a reagent comprising magnetic particles having loaded thereon a substance that binds to the target substance is utilized; non-magnetic colored particles; and magnetic particles having loaded thereon a substance that binds to an assay interfering substance is utilized. The assay is carried out by mixing the reagent with the body fluid; magnetically removing the magnetic particles and the substances that were bound to the magnetic particles; and measuring the amount of the remaining non-magnetic colored particles. Even when the body fluid specimen is measured with no pretreatment, measurements of high sensitivity and high reliability can be obtained in a short period without being interfered with the assay interfering substances in the body fluid.

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