Assay method for antibodies against cyclic citrullinated...

G - Physics – 01 – N

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G01N 33/543 (2006.01) G01N 33/564 (2006.01)

Patent

CA 2716204

The present invention relates to method for assaying anti-cyclic citrullinated peptide antibodies in a clinical sam-ple, said method comprising contacting said sample with at least one homogeneous reagent comprising at least one specific binder for anti- CCP antibodies, whereby to form a solution or suspension of an anti- CCP -binding partner complex in a homogeneous sample mixture and detecting the presence or level of said anti-CCP -binding partner complex in a homogeneous liquid-phase. The invention also relates to a method for the assessment of the existence of; risk of; potential for; or propensity to RA in a subject, said method comprising assaying for anti-CCP in a body sample from said subject in a homogeneous assay according to the invention, whereby to determine the level of anti-CCP antibodies in said sample, and correlating the thus-determined level with the existence of; risk of; potential for; or propensity to RA in said subject.

La présente invention porte sur un procédé pour doser des anticorps anti-peptide citrulliné cyclique (CCP) dans un échantillon clinique, ledit procédé comprenant la mise en contact dudit échantillon avec au moins un réactif homogène comprenant au moins un liant spécifique pour des anticorps anti-CCP, de façon à former ainsi une solution ou une suspension d'un complexe anti-CCP-partenaire de liaison dans un mélange d'échantillon homogène, et la détection de la présence ou du taux dudit complexe anti-CCP-partenaire de liaison dans une phase liquide homogène. L'invention porte également sur un procédé pour l'évaluation de l'existence, du risque, du potentiel ou de la propension à la polyarthrite rhumatoïde chez un sujet, ledit procédé comprenant le dosage d'un anti-CCP dans un échantillon corporel provenant dudit sujet lors d'un dosage homogène selon l'invention, de façon à déterminer ainsi le taux d'anticorps anti-CCP dans ledit échantillon, et la corrélation du taux ainsi déterminé avec l'existence, le risque, le potentiel ou la propension à la polyarthrite rhumatoïde chez ledit sujet.

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