Assay method for catheter related sepsis

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/04 (2006.01) G01N 33/569 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2205377

The invention provides a method of assaying a biologically active material from an in situ catheter lumen for the presence of fibrin, a microorganism and/or debris thereof which comprises sujecting the biologically active material from the in situ catheter lumen to analysis wherein the lumen is subjected to mechanical action to dislodge the biologically active material from the wall of the catheter lumen in an amount sufficient to allow identification of a target microorganism without culturing. The invention also provides an analysis kit for this purpose.

L'invention concerne une méthode destinée à analyser une matière biologiquement active à partir d'une lumière de cathéter in situ pour détecter la présence de fibrine, d'un micro-organisme et/ou de ses débris. L'opération consiste à analyser la matière biologiquement active de la lumière de cathéter in situ en soumettant la lumière à une action mécanique à même d'expulser suffisamment de matière active de la paroi du cathéter pour permettre l'identification d'un micro-organisme cible sans mise en culture. L'invention comporte également un kit d'analyse.

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