Assay method for organic calcium antagonist drugs and a kit...

G - Physics – 01 – N

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150/14

G01N 33/60 (2006.01)

Patent

CA 1252704

19 AN ASSAY METHOD FOR ORGANIC CALCIUM ANTAGONIST DRUGS AND A KIT FOR SUCH AN ASSAY ABSTRACT OF THE DISCLOSURE A method for measuring the level of organic calcium antagonist drug in a body fluid comprises preparing a mixture of a radioactive calcium antagonist drug, a body fluid containing a calcium antagonist drug and a calcium antagonist receptor material, measuring the radioactivity of the radio- active calcium antagonist drug bound to said calcium antagonist receptor material and deriving the concentration of the calcium antagonist drug in the body fluid from a standard curve indicating the concentration of calcium antagonist drug versus inhibition of binding of said radioactive calcium antagonist drug to said receptor sites caused by the calcium antagonist drug in said body fluid. A kit for measuring the level of an organic calcium drug comprises a receptacle containing a radioactive calcium antagonist drug, a calcium antagonist receptor material and a standard amount of a nonradioactive calcium antagonist drug.

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