Assay methods

Details Assay methods Assay methods

C - Chemistry, Metallurgy

12

Q

C12Q 1/37 (2006.01) A61K 31/00 (2006.01) A61K 31/165 (2006.01) A61K 31/17 (2006.01) A61K 31/18 (2006.01) A61K 31/196 (2006.01) A61K 31/395 (2006.01) A61K 31/4015 (2006.01) A61K 31/433 (2006.01) A61K 31/4412 (2006.01) A61K 31/454 (2006.01) C12N 15/11 (2006.01) G01N 33/533 (2006.01) G01N 33/566 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2393376

The invention relates to methods for identifying agents, which bind to and/or modulate exopeptidases or endopeptidases, preferably human soluble secreted endopeptidase (SEP), oxytocinase, neutral endopeptidase (NEP), or non-human SEP, preferably comprising contacting said agents with a polypeptide comprising: SEQ ID NO: 2, an amino acid sequence encoded by the polynucleotide sequence of SEQ ID NO: 1 or SEQ ID NO: 5; or a polypeptide encoded by the cDNA of NCIMB 41110, and determining whether binding and/or modulation occurs. Preferably, said method is carried out in the presence of a substrate peptide labelled with at least one fluorescent donor dye and at least one fluorescence acceptor dye, with the method used to detect said agents being a competitive binding Fluorescence Resonance Energy Transfer (FRET) assay.

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