C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/00 (2006.01) C07K 14/705 (2006.01) G01N 33/68 (2006.01)
Patent
CA 2501233
Methods of identifying activators and inhibitors of voltage-gated ion channels are provided in which the methods employ cells transfected with a voltage- gated ion channel of interest and a corollary channel to control the membrane potential of the cells by changing extracellular ion concentration. This allows for more convenient, more precise experimental manipulation of these transitions, and, coupled with efficient methods of detecting the result of ion flux through the channels, provides methods that are especially suitable for high throughput screening.
L'invention concerne des méthodes d'identification d'activateurs et d'inhibiteurs de canaux ioniques sensibles au voltage. On utilise dans lesdites méthodes des cellules transfectées par un canal ionique d'intérêt sensible au voltage et un canal secondaire pour réguler le potentiel membranaire des cellules par changement de la concentration ionique extracellulaire. Ceci permet une manipulation expérimentale plus pratique et plus précise de ces transitions, et permet d'obtenir, conjointement à des méthodes efficaces de détection du résultat du flux ionique à travers les canaux, des méthodes spécialement adaptées pour un criblage à haut rendement.
Bennett Paul B. Jr.
Cohen Charles J.
Connolly Thomas M.
Xia Menghang
Merck & Co. Inc.
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
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