Assay of igfbp complex

G - Physics – 01 – N

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G01N 33/543 (2006.01) G01N 33/74 (2006.01)

Patent

CA 2323450

A method of assaying the growth hormone status of an individual by immuno- assay for the 150KDa Insulin-like Growth Factor ternary complex (IGF/IGFBP-3/ALS). Alternatively, a binary complex may be assayed. The method involves capturing the IGFBP complex with a first antibody coupled to a solid phase and detecting the complex with a second antibody coupled to a label. A set of monoclonal and polyclonal antibodies useful for the assay is also provided.

L'invention concerne une méthode de dosage biologique du statut de l'hormone de croissance d'un individu par dosage immunologique du complexe ternaire de facteur de croissance de substances apparentées à l'insuline de 150 KDa (IGF/IGFBP-3/ALS). Dans un autre mode de réalisation, on peut effectuer un dosage biologique d'un complexe binaire. La méthode consiste à capturer le complexe d'IGFBP (protéines de liaison de facteur de croissance de substances apparentées à l'insuline) avec un premier anticorps couplé à une phase solide et à détecter le complexe avec un deuxième anticorps couplé à un marqueur. L'invention concerne également un ensemble d'anticorps monoclonaux et polyclonaux s'utilisant pour le dosage.

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