Assay of immunoreactive and like substances

Details Assay of immunoreactive and like substances Assay of immunoreactive and like substances

G - Physics

01

N

150/14, 167/44

G01N 33/546 (2006.01)

Patent

CA 1245551

ABSTRACT OF THE DISCLOSURE The disclosed methods improve conventional agglu- tination processes for assaying immunoreactive and like substances, primarily by improving the measurement of the agglutination itself. Suspensions containing agglutinated particles are automatically inspected, preferably intermit- tently during the agglutinating reaction, and the resulting data are processed to identify individual particle aggrega- tes of a selected limited class, which may, for example, comprise aggregates having sizes within a limited size interval. The numbers of such aggregates are compared with corresponding reference values obtained with standard solu- tions and suitable controls to evaluate the concentration of one of the reactive substances, or other information. The aggregate size intervals and other parameters which are used to define aggregate classes are preferably selected with attention to the detailed behavior of each test system.

471776

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