Assay of iron and iron binding protein, reagents and methods

G - Physics – 01 – N

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150/11

G01N 33/90 (2006.01)

Patent

CA 1114724

ABSTRACT Interferences encountered in the use of hemolyzed sam- ples in the process of quantitatively determining the amount of iron in a serum sample are minimized by main- taining the incubation of the sample in a buffer system for an extended period, e.g. at least about 5 minutes. The compound 9-(2-pyridyl)-acenaphtho[1,2-e]-as-triazine or its sulfonated derivative may be used as both a che- lator and indicator of ferrous ion.

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