Assay of iron and iron binding protein, reagents and methods

Details Assay of iron and iron binding protein, reagents and methods Assay of iron and iron binding protein, reagents and methods

C - Chemistry, Metallurgy

07

D

150/11, 260/248.

C07D 251/00 (2006.01) C07D 213/86 (2006.01) G01N 33/84 (2006.01)

Patent

CA 1089460

ABSTRACT The discovery of 9-(2-pyridyl)-acenaphtho [1,2-e] -as-triazine and its sulfonated form,having particular advantages when used as both a chelator and indicator of ferrous ions in organic or aqueous mediums; a process for the synthesis of those com- pounds in which hydrzaine is used as both a reactant and as a solvent in production of intermediate com- pounds, eliminating the need of ethanol and the disad- vantageous ether-extraction step; the use of N,N- dimethylformamide as a reaction solvent in the produc- tion of triazines; the use of the new triazine com- pounds to measure iron, transferrin, and UIBC/TIBC in biological systems; the use of dimethylsulfoxide and certain processes to eliminate interferences commonly encountered in these assays; and the use of the triazine compounds to measure both total and/or reduced iron in a variety of matrices.

275925

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