Assay of sequences using amplified genes

Details Assay of sequences using amplified genes Assay of sequences using amplified genes

C - Chemistry, Metallurgy

12

Q

150/8.5

C12Q 1/68 (2006.01)

Patent

CA 1317535

ABSTRACT OF THE DISCLOSURE A method for amplifying specific target nucleic acid sequences in a sample, the method comprising (a) contacting under hybridization conditions (i) two different immobilized or immobilizable primer nucleic acids, with (ii) a test sample containing target nucleic acid sequences, (b) contacting the resultant product of step (a) with an extender enzyme and at least one nucleic acid residue under conditions to elongate the resultant hybridized primer nucleic acids, and (c) denaturing the product of step (b), to produce immobilized target nucleic acid sequences, and (d) repeating at least once a cycle of steps (a), (b) and (c) with the product of step (c) of the previous cycle being used in place of (a)(ii). The above described method can be used to detect specific target nucleic acid sequences by using a test sample suspected of containing target nucleic acid sequences and determining if an amplified sequence is present.

568949

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