Assay of vitamin b in12 xx

Details Assay of vitamin b in12 xx Assay of vitamin b in12 xx

G - Physics

01

N

150/11

G01N 33/48 (2006.01) G01N 33/72 (2006.01) G01N 33/80 (2006.01) G01N 33/82 (2006.01) G01N 33/86 (2006.01) G01N 33/96 (2006.01)

Patent

CA 1104911

Abstract The amount of vitamin B12 in solution in a liquid is determined by mixing a sample of the solution with vitamin B12-binding protein from chicken serum in a pH of about 12.8 to 13.2, and then analysing a com- ponent of the mixture. In preferred procedures, especially useful in assaying human sera, a competitive binding technique is employed utilising labelled vitamin B12 and a buffer containing cyanide ion. By operating at very high pH and with chicken serum proteins, the assay can be conducted relatively simply and with very accurate results.

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