Assay reagents

Details Assay reagents Assay reagents

C - Chemistry, Metallurgy

12

Q

150/15.1, 150/3.

C12Q 1/28 (2006.01) C12N 9/96 (2006.01) G01N 33/535 (2006.01)

Patent

CA 1277895

ABSTRACT: IMPROVEMENTS RELATING TO ASSAY REAGENTS Improved peroxidase-containing and tetramethylbenzidine peroxidase-substrate reagents. The peroxidase-containing reagent consists of a buffered aqueous solution comprising a peroxidase, such as horseradish peroxidase, or a peroxidase conjugate, and a specific substrate for the peroxidase, such as tetramethylbenzidine, in the absence of peroxide. The peroxidase conjugate is suitably a conjugate of a peroxidase and a specific binding component of an assay. The tetramethylbenzidine peroxidase-substrate reagent comprises tetramethylbenzidine and .beta.-cyclodextrin in aqueous solution. The solution may additionally contain a peroxide. One or both improved reagents may be included in an assay reagent kit.

503523

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