Assay system with in situ formation of diazo reagent

Details Assay system with in situ formation of diazo reagent Assay system with in situ formation of diazo reagent

G - Physics

01

N

G01N 33/72 (2006.01)

Patent

CA 2569206

A total bilirubin diazo assay is disclosed in which an acidic solution containing a primary aryl amine is combined directly with a nitrite-containing sample solution at approximately neutral pH, resulting in the in situ generation of a diazo compound in the sample solution. The diazo compound reacts with bilirubin in the sample solution to form a colored reaction product that can be measured.

L'invention concerne un dosage total de diazo-bilirubine : une solution acide contenant une aryle amine primaire est combinée directement avec une solution d'échantillon contenant nitrite à un pH approximativement neutre, ce qui permet de former in situ un composé diazo dans ladite solution. Le composé diazo réagit avec la bilirubine dans la solution pour former un produit de réaction coloré pouvant être mesuré.

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