G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/542 (2006.01) G01N 33/68 (2006.01)
Patent
CA 2574921
The invention provides an assay system for detecting a molecule that interacts with a membrane channel, the assay system including cell membranes including one or more membrane channels; support bodies including scintillant and a coupling agent that associates with the cell membrane; a ligand that is selected to bind the membrane channel, the ligand including a scintillant- activating label. According to the invention, association of the support bodies with the cell membrane and binding of the ligand to the membrane channel results in emission from the scintillant of the support bodies, and, in the presence of a test molecule that interacts with the membrane channel, the emission from the scintillant of the support bodies changes. Methods of identifying a molecule that interacts with a cell membrane channel are also described.
L'invention concerne un système de dosage biologique destiné à détecter une molécule interagissant avec un canal membranaire. Ledit système de dosage biologique comporte des membranes cellulaires contenant un ou plusieurs canaux membranaires ; des corps support contenant un agent scintillant et un agent de couplage s'associant à la membrane cellulaire ; et un ligand sélectionné pour lier le canal membranaire, contenant une étiquette d'activation d'agent scintillant. Selon l'invention, l'association des corps support avec les membranes cellulaires et la liaison du ligand à un canal membranaire se traduisent par une émission par l'agent scintillant des corps support, et en présence d'une molécule de test interagissant avec le canal membranaire, l'émission par l'agent scintillant des corps support change. L'invention concerne également des procédés d'identification d'une molécule interagissant avec un canal membranaire cellulaire.
Codd Ellen E.
Zhang Sui-Po
Janssen Pharmaceutica N.v.
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
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