Assay technique

Details Assay technique Assay technique

G - Physics

01

N

167/43, 150/8

G01N 21/25 (2006.01) G01N 21/47 (2006.01) G01N 33/543 (2006.01)

Patent

CA 1237645

A B S T R A C T "ASSAY TECHNIQUE" An assay technique is disclosed for the qualitative and/or quantitative detection of a chemical, biochemical or biological species in a sample. The technique comprises (a) coating at least a predetermined part of a pre-formed surface on a substrate with a thin film of a material capable of binding the species to be assayed, the pre-formed surface being optically active with respect to radiation at least over a predetermined band of wavelengths; (b) contacting the coated surface with the sample; and (c) observing the optical properties of said pre-formed surface in order to determine a qualitative and/or quantitative change in optical properties as a result of the binding of the species onto said thin film of material. The optical properties of the pre-formed surface may be observed before and after step (b) in order to determine any change in optical properties, or they may be monitored during step (b). The pre-formed surface is preferably a grating. An article for use in the above technique is also disclosed, and comprises a substrate carrying said pre-formed surface which in turn is coated with the receptive material for the species to be assayed.

443968

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