G - Physics – 01 – N
Patent
G - Physics
01
N
150/15, 324/23
G01N 33/53 (2006.01) A61B 5/00 (2006.01) C07F 17/02 (2006.01) C12Q 1/00 (2006.01) C12Q 1/68 (2006.01) G01N 33/543 (2006.01)
Patent
CA 1220818
ABSTRACT OF THE DISCLOSURE The specification relates to, immunoassay techniques for ligands and analytes, including both antigens and haptens on a carrier, and, types of reactions in which specific, non-immunological binding occurs, such as with nucleic acids. The specification further relates the use of mediator compounds to transfer charge from an enzyme to an electrode when the electrode is catalytically active. The invention concerns, an assay system comprising: (a) a ligand-antiligand pair or analyte-specific binding agent pair capable of specifically binding together, (T4A); (b) a mediator compound (F) enzyme (GOD) and corresponding substrate (GLUCOSE) triplet, whereby conversion of the substrate into a product by the enzyme generates electrical charge transferred via the mediator to an electrode; wherein at least some of one of the pair and at least some of one of the triplet are chemically cross-linked (GOD-T4), so as to inhibit at least a part of the said charge transfer when the ligand and antiligand pair or analyte-specific binding agent pair are bound together and, wherein the addition of either one of the said pair or one of the said triplet changes the rate of charge transfer to the electrode. The invention further concerns equipment or materials useful in the above techniques.
453584
Genetics International Inc.
Marks & Clerk
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