G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/574 (2006.01) G01N 33/543 (2006.01) C12Q 1/48 (2006.01)
Patent
CA 2676478
A lipid assay method, kit, and apparatus involving exposure of a protein, having a lipid recognition motif that interacts with a target lipid and a competing lipid, to a solution containing the competing lipid, and determining whether the target lipid is present in the solution. The target lipid has a stronger affinity to the lipid recognition motif than does the competing lipid. The lipid recognition motif is preferably a pleckstrin homology (PH) domain, with the target lipid being a phosphoinositide. The assay determines activity of a lipid kinase, the target lipid being a phosphorylation product of a reaction between the lipid kinase and a substrate lipid. The assay can be a cancer screening method for detection of cancer cells, where detection of certain levels of a PI(3,4,5)P3 target lipid is an indicator of a cancer cell.
Chakravarty Leena
Drees Beth E.
Mostert Michael J.
Neilsen Paul O.
Prestwich Glenn
Echelon Biosciences Inc.
Smart & Biggar
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