Assaying nucleotides in solution using pna probes

Details Assaying nucleotides in solution using pna probes Assaying nucleotides in solution using pna probes

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2282704

The invention provides a method for rapidly, economically and efficiently sequencing and assaying nucleotides in a liquid medium using PNA probes. The method is particularly advantageous in not requiring the use of a solid support to bind the probe or the target sequence. The method is capable of providing information regarding the nature and existence of hybridization.

L'invention fournit une méthode qui permet un séquençage et un dosage rapides, économiques et efficaces de nucléotides dans un milieu liquide, à l'aide de sondes d'acides nucléiques peptidiques (PNA). La méthode convient particulièrement puisqu'elle ne nécessite pas l'utilisation d'un support solide pour lier la sonde ou la séquence cible. La méthode peut fournir des informations sur la nature et l'existence d'une hybridation.

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