Assays

B - Operations – Transporting – 01 – L

Patent

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Details

B01L 3/00 (2006.01)

Patent

CA 2722242

A device comprising a rigid substrate, a flexible cover element at least partially covering the substrate, a first structure formed in the substrate, adapted for accommodating liquids and adapted for releasing contents of one or more cells, spores, or viruses, the contents including the target molecules, a second structure formed in the substrate, adapted for accommodating liquids and comprising at least one binding member adapted for capturing the target molecules and for determining a value indicative for the presence and/or amount of the target molecules, a microfluidic network interconnecting at least the first structure and the second structure, and an actuator member adapted for effecting a fluid flow between the first structure and the second structure by pressing the flexible cover element against the substrate to selectively close a portion of the microfluidic network.

La présente invention concerne un dispositif comprenant un substrat rigide, un élément de couverture flexible couvrant au moins partiellement le substrat, une première structure formée dans le substrat, adaptée pour contenir des liquides et adaptée pour libérer les contenus d'une ou des cellules, spores, or virus, les contenus comprenant les molécules cibles, une seconde structure formée dans le substrat, adaptée pour contenir des liquides et comprenant au moins un élément de liaison adapté pour capturer les molécules cibles et pour déterminer une valeur indicatrice de la présence et/ou de la quantité des molécules cibles, un réseau microfluidique interconnectant au moins la première structure et la seconde structure, et un élément actionneur adapté pour mettre en place un écoulement de fluide entre la première structure et la seconde structure en appuyant l'élément de couverture flexible contre le substrat pour fermer de manière sélective une partie du réseau microfluidique.

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