Assays and methods using biomarkers

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2575755

Methods and assays examining expression of one or more biomarkers in a mammalian tissue or cell sample are provided. According to the disclosed methods and assays, detection of the expression of one or more such biomarkers is predictive or indicative that the tissue or cell sample will be sensitive to apoptosis-inducing agents such as Apo2L/TRAIL and anti-DR5 agonist antibodies. Certain biomarkers which may be examined include fucosyltransferases, in particular fucosyltransferase 3 (FUT3) and/or fucosyltransferase 6 (FUT6), as well as sialyl Lewis A and/or X antigens. Kits and articles of manufacture are also provided.

La présente invention a trait à des procédés et des dosages d'examen de l'expression d'un ou de plusieurs biomarqueurs dans un prélèvement tissulaire ou cellulaire mammalien. Selon les procédés et dosages de l'invention, la détection de l'expression d'un ou de plusieurs tels biomarqueurs constitue une prévision ou une indication que le prélèvement tissulaire ou cellulaire va être sensible à des agents d'induction de l'apoptose tels que Apo2L/TRAIL et des anticorps agonistes anti-DR5. Certains biomarqueurs qui peuvent être observés comprennent des fucosyltransférases, notamment la fucosyltransférase 3 (FUT3) et/ou la fucosyltransférase 6 (FUT6), ainsi que des antigènes de sialyle Lewis A et/ou X. L'invention a également trait à des trousses et des articles de fabrication.

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