Assays and methods using biomarkers

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2575152

Methods and assays examining expression of one or more biomarkers in a mammalian tissue or cell sample are provided. According to the disclosed methods and assays, detection of the expression of one or more such biomarkers is predictive or indicative that the tissue or cell sample will be sensitive to apoptosis-inducing agents such as Apo2L/TRAIL and anti-DR5 agonist antibodies. Certain biomarkers which may be examined include fucosyltransferases, in particular fucosyltransferase 3 (FUT3) and/or fucosyltransferase 6 (FUT6), as well as sialyl Lewis A and/or X antigens. Kits and articles of manufacture are also provided.

L'invention concerne de méthodes et des tests permettant de détecter l'expression d'un ou de plusieurs marqueurs biologiques dans échantillon tissulaire ou cellulaire de mammifère. Dans ces méthodes et ces tests, la détection de l'expression d'un ou de plusieurs marqueurs biologiques prédit ou indique que l'échantillon tissulaire ou cellulaire sera sensible à des agents induisant l'apoptose tels que Apo2L/TRAIL et les anticorps dirigé contre les agonistes DR5. Certains marqueurs biologiques examinés comprennent de fucosyltransférases, en particulier les fucosyltransférase 3 (FUT3) et/ou les fucosyltransférase 6 (FUT6), ainsi que des antigènes sialyl Lewis A et/ou X. L'invention concerne également des trousses de matériel et des articles de fabrication.

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