Assays for caspase activity using green fluorescent proteins

Details Assays for caspase activity using green fluorescent proteins Assays for caspase activity using green fluorescent proteins

C - Chemistry, Metallurgy

12

N

C12N 15/12 (2006.01) C07K 14/435 (2006.01) C12N 9/64 (2006.01) C12Q 1/37 (2006.01) G01N 33/50 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2374369

Provided are substrates and assays for the identification of caspase activators and inhibitors. The substrates are fusion proteins comprising two green fluorescent proteins (GFPs) with a linker peptide comprising at least one caspase cleavage site. The intact fusion protein exhibits fluorescent resonance energy transfer (FRET) between the GFPs. Following caspase cleavage of the linker peptide, the two GFPs become separated and FRET is diminished.

Substrats et analyses permettant l'identification d'activateurs et d'inhibiteurs de caspases. Lesdits substrats sont des protéines de fusion comprenant deux protéines fluorescentes vertes (GFPs) avec un peptide de liaison comportant au moins un site de clivage par caspase. La protéine de fusion intacte présente un transfert d'énergie de fluorescence par résonance (FRET) entre les GFPs. Suite au clivage par caspase du peptide de liaison, les deux GFPs se séparent et le FRET s'en trouve réduit.

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