Assays for detecting, identifying and/or quantifying...

G - Physics – 01 – N

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G01N 33/543 (2006.01)

Patent

CA 2454470

An assay for detecting, identifying and/or quantifying at least one contaminant in a sample comprising (a) mixing the sample with liposomes having a known capture efficiency so as to form a mixture; (b) isolating the liposomes including the contaminant bound liposomes from the mixtures; (c) detecting and quantifying the captured contaminant; (d) determining the concentration of the contaminant in the sample by multiplying the liposomes' capture efficiency with the quantity of contaminant determined in (c), wherein the lipid composition of the liposomes possesses affinities with said at least one contaminant so that if the sample contains contaminants, at least a portion thereof is bound to or captured by the liposomes. The contaminants can be isolated and even concentrated in the liposomes during the assay process. The assays of the present invention are advantageously simple and inexpensive to perform and are particularly suitable for tests performed outside of sophisticated laboratories.

L'invention concerne un procédé de dosage destiné à identifier et/ou quantifier au moins un contaminant dans un échantillon. Le procédé consiste à: a) mélanger l'échantillon avec des liposomes ayant un rendement de captation connu, de façon à former un mélange; b) isoler desdits mélanges les liposomes comprenant les liposomes liés au contaminant; c) détecter et quantifier le contaminant piégé; d) déterminer la concentration du contaminant dans l'échantillon en multipliant le rendement de captation avec la quantité de contaminant déterminé à l'étape c), la composition lipidique des liposomes possédant des affinités pour ledit contaminant si bien que, lorsque l'échantillon contient des contaminants, au moins une partie de ceux-ci est liée à ou piégée par les liposomes. Les contaminants peuvent être isolés ou même concentrés dans les liposomes pendant le processus de dosage. Les dosages de l'invention sont avantageux en ce qu'il peuvent être réalisés de manière simple et bon marché, et conviennent particulièrement pour des essais devant être effectués hors de laboratoires perfectionnés.

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