Assays for detecting modulators of cytoskeletal function

Details Assays for detecting modulators of cytoskeletal function Assays for detecting modulators of cytoskeletal function

C - Chemistry, Metallurgy

12

Q

C12Q 1/00 (2006.01) C12Q 1/42 (2006.01) G01N 31/00 (2006.01) G01N 33/53 (2006.01) G01N 33/542 (2006.01) G01N 33/543 (2006.01) G01N 33/544 (2006.01) G01N 33/545 (2006.01) G01N 33/567 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2300869

Described herein are methods of identifying compounds which modulate the activity of the cytoskeletal system. The methods are rapid, convenient and sensitive. Preferably, the method is used to identify lead compounds that can be used as therapeutics, diagnostics and agricultural agents. Generally, test compounds are added to two cytoskeletal components which bind to one another, to determine whether the binding is affected by the test compound. Wherein the binding is affected, a compound which modulates the cytoskeletal system is identified.

L'invention se rapporte à des méthodes d'identification de composés qui modulent l'activité du système cytosquelettique. Ces méthodes sont rapides, efficaces et sensibles. De préférence, ces méthodes visent à identifier des composés de plomb susceptibles d'être utilisés en tant qu'agents thérapeutiques, diagnostiques et agents destinés à l'agriculture. Généralement, on ajoute les composés d'essai à deux composants qui se lient l'un à l'autre, de façon à évaluer l'influence du composé d'essai sur la liaison. Lorsque cette liaison est influencée par le composé d'essai, on en déduit que ce composé est un composé modulateur du système cytosquettique.

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