Assays for measurement of type ii collagen fragments in urine

Details Assays for measurement of type ii collagen fragments in urine Assays for measurement of type ii collagen fragments in urine

G - Physics

01

N

G01N 33/577 (2006.01) G01N 33/53 (2006.01) G01N 33/566 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2332132

Disclosed is a novel method for monitoring urine for type II collagen fragments. The method comprises a) contacting the urine with a capture antibody, wherein the capture antibody binds specifically to type II collagen fragments, to the substantial exclusion of any binding to type I or III collagen fragments; b) contacting the urine with a detection antibody, wherein the detection antibody binds specifically to collagenase-generated collagen fragments; and c) detecting the amount of type II collagen fragments bound to the capture and detection antibodies. Also disclosed are a kit and a biosensor chip adapted for use in the method.

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