Assays for myasthenia gravis and substrates and kits for use...

G - Physics – 01 – N

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G01N 33/567 (2006.01) G01N 33/536 (2006.01) G01N 33/564 (2006.01)

Patent

CA 1287797

ABSTRACT IMPROVED ASSAYS FOR MYASTHENIA GRAVIS AND SUBSTRATES AND KITS FOR USE THEREIN Improved assays are provided for anti-acetylcholine receptor protein autoantibodies in the sera of patients with myasthenia gravis. The basis for the improved assays is the discovery that the acetylcholine receptor protein, that can be isolated in large quantities from the human medullablastoma-derived cell line TE671, is, for practical purposes in immunoassays, immunologically indistinguishable from human muscle acetylcholine receptor protein. The discovery makes practicable solid-phase immunoassays for serum anti-acetycholine receptor protein autoantibodies; accordingly the invention provides solid supports with the TE671 line-derived acetylcholine receptor protein affixed thereto for use as substrates in such solid-phase immunoassays and kits for carrying out such immunoassays with such substrates.

526741

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