Assays for nucleoside diphosphates and triphosphates

Details Assays for nucleoside diphosphates and triphosphates Assays for nucleoside diphosphates and triphosphates

C - Chemistry, Metallurgy

12

N

C12N 9/12 (2006.01) A61K 49/00 (2006.01) A61K 51/08 (2006.01) C12N 11/00 (2006.01) C12Q 1/48 (2006.01)

Patent

CA 2371176

Nucleoside diphosphates (NDPs) are detected or measured by following the dephosphorylation of the phosphoenzyme form of nucleoside diphosphate kinase (NDPK), and nucleoside triphosphates (NTPs) are detected or measured by following the phosphorylation of NDPK to its phosphoenozyme form. A typical process involves (a) causing NDP in sample to bind to NDPK phosphoenzyme, or causing NTP in sample to phosphorylate NDPK and (b) detecting a change in a charateristic of the enzyme which differs between its phosphorylated and unphosphorylated forms. This may be aided by labelling the enzyme. Quantitative data can be obtained. Both in vivo and in vitro measurements can be made.

Des nucléosides diphosphates (NDP) sont détectés ou mesurés en suivant la déphosphorylation de la forme phosphoenzyme de la nucléoside diphosphate kinase (NDPK), et des nucléosides diphosphates (NDP) sont détectés ou mesurés en suivant la phosphorylation de la NDPK dans sa forme phosphoenzyme. Un procédé traditionnel consiste à (a) amener le NDP dans l'échantillon à se lier au phosphoenzyme de NDPK, ou amener le NTP dans l'échantillon à se phosphoryler en NDPK et (b) à détecter un changement de caractéristique de l'enzyme qui diffère entre ses formes phosphorylée et non phosphorylée. Ce procédé peut être facilité en étiquetant l'enzyme. Des données quantitatives peuvent être obtenues. Il est possible de réaliser des mesures in vivo et in vitro.

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