Assays for the identification of modulators of mhc class ii...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2565762

The present invention provides methods for accurate and efficient detection of compositions that specifically modulate the interaction and/or activity of CIITA, RFXANK, RFXAP and RFX5 polypeptide factors. Further methods are provided for identifying compositions whose specific modulation of these transcription factors affects expression of a MHC class II promoter. The disclosed methods are configured in an assay format useful in moderate and high throughput screening applications.

La présente invention concerne des procédés de détection précise et efficace de compositions modulant spécifiquement l'interaction et/ou l'activité des facteurs polypeptidiques CIITA, RFXANK, RFXAP et RFX5. D'autres procédés sont prévus pour identifier des compositions dont la modulation spécifique de ces facteurs de transcription influe sur l'expression d'un promoteur de CMH classe II. Les procédés décrits sont configurés en un format de dosage utile dans des applications de criblage à rendement modéré et élevé.

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