Assays of molecular or subcellular interactivity using...

G - Physics – 01 – N

Patent

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G01N 33/542 (2006.01)

Patent

CA 2602972

The present invention provides a method of determining the proximity, or changes in the proximity of two or more molecular features labeled with fluorophores able to undergo DARET. In preferred embodiments the change in proximity of molecular features in a sample is correlated with changes in the fluorescence polarization of the sample, and may be monitored in real time.

La présente invention concerne un procédé pour déterminer la proximité ou des variations dans la proximité d'au moins deux caractéristiques moléculaires marquées avec des fluorophores capables de subir une dépolarisation après transfert d'énergie de résonance (DARET). Dans des modes de réalisation préférés, la variation de proximité de caractéristiques moléculaires dans un échantillon, est mise en corrélation avec des variations de la polarisation de fluorescence de l'échantillon, et peut être suivie en temps réel.

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