Assays using albumin-tetrazolium interaction

G - Physics – 01 – N

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G01N 33/68 (2006.01) C07C 245/12 (2006.01) G01N 33/52 (2006.01)

Patent

CA 2050884

2050884 9012318 PCTABS00002 A method for the reduction of a tetrazolium compound containing the cationic nucleus (I), to produce a ring - opened formazan compound containing the group (II), in which the tetrazolium compound is caused to interact with albumin and the tetrazolium compound - albumin ineraction product is reduced with a reducing agent in an aqueous solution at a pH between 6 and 10. The method is applied to the assay of albumin and reducing agents in samples, in which the amount of formazan produced is related to the presence and/or amount of albumin or reducing agent present.

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