Assays utilizing sensitizer-induced production of detectable...

G - Physics – 01 – N

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167/43, 358/5, 1

G01N 33/58 (2006.01) C07H 21/00 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) G01N 33/68 (2006.01)

Patent

CA 1338927

Specific binding assays are disclosed which utilize a sensitizer as a label. Such sensitizers include any moiety which, when stimulated by "excitation" wlth radiation of one or more wavelengths or other chemical or physical stimulus (e.g., electron transfer, eLectrolysis, electroluminescence or energy transfer), will achieve an excited state which (a) upon interaction with molecular oxygen will produce singlet molecular oxygen, or (b) upon interaction with a leucodye will assume a reduced form which can then be returned to its original unexcited state by interaction with molecular oxygen resulting in the production of hydrogen peroxide. Either interaction with the excited sensitizer will, with the addition of other reagents, produce a detectable signal.

601917

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