Assembly activating protein (aap) and its use for the...

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 14/015 (2006.01) A61K 39/23 (2006.01) C12N 7/04 (2006.01) C12N 15/35 (2006.01)

Patent

CA 2754335

The present invention relates to nucleic acids encoding the novel parvoviral protein "assembly activating protein" (AAP), the encoded polypeptides, methods of producing the polypeptides, antibodies specific for AAP, the use of the nucleic acids for the preparation of the polypeptides, the use of the nucleic acids or the polypeptides for the preparation of the parvoviral particle and methods of producing parvoviral particles essentially consisting of VP3 by providing in addition to the coding sequence of the parvoviral structural protein VP3 a sequence fragment Z/a nucleic acid encoding AAP in the cell and expressing VP3 and fragment Z under control of a rep- independent promoter. Furthermore, the present invention relates to parvoviral particles essentially consisting of VP3 and/or obtainable by the above method as well as expression cassettes comprising (i) a heterologous promoter and (ii) VP3 coding sequence and/or fragment Z. The present invention further relates to a medicament, particularly a vaccine, comprising the parvoviral particles or expression cassettes and their use.

La présente invention concerne des acides nucléiques codant la nouvelle protéine parvovirale « protéine activant l'assemblage » (AAP), les polypeptides codés, des procédés de production des polypeptides, des anticorps spécifiques de l'AAP, l'utilisation des acides nucléiques pour la préparation des polypeptides, l'utilisation des acides nucléiques ou des polypeptides pour la préparation des particules parvovirales et des procédés de production de particules parvovirales consistant essentiellement en VP3, en fournissant, en plus de la séquence codante de la protéine structurale parvovirale VP3, un fragment de séquence Z/un acide nucléique codant AAP dans la cellule et exprimant VP3 et le fragment Z sous le contrôle d'un promoteur rep-indépendant. En outre, la présente invention concerne des particules parvovirales consistant essentiellement en VP3 et/ou pouvant être obtenues par le procédé ci-dessus ainsi que des cassettes d'expression comprenant (i) un promoteur hétérologue et (ii) une séquence codant VP3 et/ou un fragment Z. La présente invention concerne en outre un médicament, en particulier un vaccin, comprenant les particules parvovirales ou des cassettes d'expression et leur utilisation.

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