Assembly of large nucleic acids

C - Chemistry – Metallurgy – 12 – P

Patent

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Details

C12P 19/34 (2006.01) C12N 15/09 (2006.01) C12N 15/81 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2701923

A method to assemble any desired nucleic acid molecule by combining cassettes in vitro to form assemblies which are further combined in vivo, or by assembling large numbers of DNA fragments by recombination in a yeast culture to obtain desired DNA molecules of substantial size is described.

L'invention concerne un procédé d'assemblage de toute molécule d'acide nucléique souhaitée par combinaison de cassettes in vitro pour former des assemblages qui sont encore combinés in vivo, ou par assemblage de grand nombre de fragments d'ADN par recombinaison dans une culture de levure pour obtenir les molécules d'ADN de taille substantielle souhaitées.

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