Asymmetric cyanine dye quenchers

Details Asymmetric cyanine dye quenchers Asymmetric cyanine dye quenchers

C - Chemistry, Metallurgy

09

B

C09B 23/02 (2006.01) C07H 19/04 (2006.01) C07H 21/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2318160

The invention provides an asymmetric cyanine dye compound having structure (1) including substituted forms thereof, wherein, at least one of R1 and R2 is linking group. X is O, S, or Se, and n ranges from 0 to 2. The invention further provides reporter-quencher dye pairs comprising the asymmetric cyanine dyes, dye-labeled polynucleotides incorporating the asymmetric cyanine dyes, and hybridization detection methods utilizing the dye-labeled polynucleotides.

Cette invention concerne un composé de colorant cyanine asymétrique correspondant à la formule (1), ainsi que ses formes substituées. Dans la formule, au moins un élément entre R1 et R2 représente un groupe de liaison, X représente O, S ou Se et n est compris entre 0 et 2. Cette invention concerne également des paires de colorants marqueur-extincteur comprenant les colorants cyanine asymétriques, ainsi que des procédés de détection d'hybridation dans lesquels on utilise les polynucléotides marqués par colorant.

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