Atopic dermatitis inducer

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 14/47 (2006.01) A61K 49/00 (2006.01) A61P 37/08 (2006.01) C07K 16/18 (2006.01)

Patent

CA 2536354

A purified human secretion fraction, which is an atopic dermatitis inducer binding to human IgE antibody per se and activating mast cells and basophiles, obtained by the following steps: the step of filtering a human secretion, removing the insoluble matters and collecting the filtrate; the step of mixing the filtrate with a ConA-affinity carrier and collecting the supernatant; and the step of separating a component showing a histamine release activity form the supernatant by column chromatography; or an antigen molecule or an antigen determinant contained in this purified fraction. This inducer is efficacious in diagnosing and treating human atopic dermatitis.

L'invention porte sur une fraction de sécrétion humaine purifiée possédant un inducteur de dermatite atopique se liant à un anticorps IgE humain per se et activant les mastocytes et les basophiles, est obtenue au cours des étapes suivantes consistant : à filtrer une sécrétion humaine, à ôter les matières insolubles et à recueillir le filtrat ; à mélanger le filtrat à un support d'affinité à ConA et à recueillir le surnageant ; et à séparer un composant possédant une activité de libération d'histamine à partir du surnageant par chromatographie sur colonne ; ou sur une molécule d'antigène ou un déterminant d'antigène contenu dans cette fraction purifiée. Cet inducteur est efficace dans le diagnostic et le traitement de la dermatite atopique humaine.

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