C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C07H 19/04 (2006.01) C07H 19/10 (2006.01) C07H 19/20 (2006.01) C07H 21/00 (2006.01) C09B 11/04 (2006.01) G01N 33/533 (2006.01) G01N 33/58 (2006.01)
Patent
CA 2426121
Substantially pure atropisomers of xanthene compounds are disclosed. A variety of molecular biology applications utilize atropisomeric xanthene fluorescent dyes as labels for substrates such as nucleotides, nucleosides, polynucleotides, polypeptides and carbohydrates. Methods include DNA sequencing, DNA fragment analysis, PCR, SNP analysis, oligonucleotide ligation, amplification, minisequencing, and primer extension.
Cette invention concerne des atropisomères sensiblement purs de composés de xanthène. En biologie moléculaire, diverses applications font intervenir des teintures fluorescentes de xanthène atropisomère comme marqueurs de divers substrats : nucléotides, nucléosides, polynucléotides, polypeptides et glucides notamment. Ces méthodes englobent le séquençage d'ADN, l'analyse de fragments d'ADN, la réaction en chaîne de la polymérase (PCR), l'analyse du ploymorphisme d'un seul nucléotide (SNP), la ligation d'oligonucléotides, l'amplification, le miniséquençage et l'allongement d'amorce.
Lee Linda G.
Rosenblum Barnett B.
Taing Meng C.
Applera Corporation
Lee Linda G.
Rosenblum Barnett B.
Sim & Mcburney
Taing Meng C.
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