C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C07B 61/00 (2006.01) C07C 47/575 (2006.01) C07C 49/84 (2006.01) C07C 281/06 (2006.01) C07F 9/24 (2006.01) C07H 21/00 (2006.01)
Patent
CA 2379828
Derivatized oligonucleotides (ODNs) are coupled to a solid support in improved yield resulting in a high density of coupled oligonucleotide per surface unit of the support, through a Schiff base type bond formed between an NH2 group attached either to the solid support or to the ODN and an aromatic aldehyde attached to the other of the solid support and the ODN. The preferred solid support-ODN conjugate is formed between semicarbazide groups attached to a glasss surface and an aromatic aldehyde attached at either 3', or 5' end of an ODN or to an intermediate nucleotide of the ODN.
L'invention concerne des oligonucléotides dérivatisés (ODN) sont couplés à un support solide à meilleur rendement, produisant une densité élevée d'oligonucléotides couplés par unité de surface du support, au moyen d'une liaison de type base de Schiff formée entre un groupe NH¿2? fixé soit au support solide soit à l'ODN et un aldéhyde aromatique fixé à l'élément restant. Le conjugué support solide-ODN est formé entre des groupes semicarbazides fixés à une surface en verre et un aldéhyde aromatique fixé à l'extrémité 3' ou 5' d'un ODN ou à un nucléotide intermédiaire de l'ODN.
Hedgpeth Joel
Lukhtanov Eugeny Alexander
Podyminogin Mikhail A.
Epoch Biosciences Inc.
Fetherstonhaugh & Co.
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