Attenuated oncolytic paramyxoviruses encoding avian cytokines

A - Human Necessities – 61 – K

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A61K 35/76 (2006.01)

Patent

CA 2711571

The invention refers to a recombinant oncolytic RNA Newcastle Disease Virus for the treatment of a proliferative disease, comprising at least one transgene coding for an avian cytokine, wherein the recombinant oncolytic RNA Newcastle Disease Virus is obtainable from a velogenic or mesogenic oncolytic RNA Newcastle Disease Virus. Virus-mediated expression of the cytokine in the natural host cells leads to a reduced pathogenicity of the virus for avian species. Furthermore the virus genome can encode binding proteins, prodrug-converting enzymes or/and proteases. The selective expression of these molecules in virus-infected tumor cells increases the anti-tumor effect of the virus.

L'invention porte sur un virus de la maladie de Newcastle à ARN oncolytique recombiné pour le traitement d'une maladie proliférative. Ce virus comprend au moins un transgène codant pour une cytokine aviaire, le virus de la maladie de Newcastle à ARN oncolytique recombiné pouvant être obtenu à partir d'un virus de la maladie de Newcastle à ARN oncolytique vélogénique ou mésogénique. L'expression à médiation par le virus de la cytokine dans les cellules hôtes naturelles conduit à une pathogénicité réduite du virus pour les espèces aviaires. De plus, le génome du virus peut coder pour des protéines de liaison, des enzymes de conversion de promédicament et/ou des protéases. L'expression sélective de ces molécules dans des cellules tumorales infectées par un virus augmente l'effet antitumoral du virus.

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