Attenuated reovirus

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 7/01 (2006.01) A61K 35/76 (2006.01) A61P 35/00 (2006.01) C07K 14/14 (2006.01) C12N 7/04 (2006.01) C12N 15/46 (2006.01) C12N 15/86 (2006.01) A61K 39/15 (2006.01)

Patent

CA 2617600

Compositions and methods are provided that relate to an attenuated reovirus exhibiting oncolytic activity toward cancer cells while displaying reduced lytic activity toward non-malignant cells. Exemplified is an attenuated human reovirus derived from persistently infected fibrosarcoma cells that lacks wild- type reovirus S1 and S4 genes and consequently lacks a detectable reoviral outer capsid .sigma.l protein and expresses a mutated reoviral outer capsid .sigma.3 protein.

La présente invention concerne des compositions et des procédés relatifs à un réovirus atténué qui possède une activité oncolytique vis-à-vis de cellules cancéreuses tout en présentant une activité lytique réduite vis-à-vis de cellules non malignes. Dans un exemple de la présente invention est décrit un réovirus humain atténué dérivé de cellules de fibrosarcome constamment infectées, qui est dépourvu des gènes S1 et S4 du réovirus de type sauvage et qui, par conséquent, est dépourvu d'une protéine réovirale .sigma.l détectable de la capside externe et exprime une protéine réovirale .sigma.3 mutée de la capside externe.

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