A - Human Necessities – 61 – L
Patent
A - Human Necessities
61
L
A61L 27/38 (2006.01) C12N 5/071 (2010.01) A61F 2/02 (2006.01) A61F 2/10 (2006.01) A61F 2/20 (2006.01) A61L 27/24 (2006.01) A61L 27/56 (2006.01) A61M 5/178 (2006.01) A61K 35/12 (2006.01) C12N 11/02 (2006.01)
Patent
CA 2281758
This invention is a methodology for the long-term augmentation, and/or repair of dermal, subcutaneous, or vocal cord tissue by the injection, or direct surgical placement of autologous cultured fibroblasts derived from connective tissue, or dermis, or fascia lamina propria tissue fibroblasts derived from the lamina propria or adipocytes. The fibroblast cultures utilized for the augmentation, and/or repair of skin defects are derived from either connective tissue, dermal, and/or fascial fibroblasts. In addition a methodology of rendering the cultured cells substantially free of potentially immunogenic serum derived proteins by late stage passage of the cultured fibroblasts lamina propria tissue, or adipocytes in serum free medium in the patient's own serum.
La présent invention concerne une méthodologie d'augmentation à long terme et/ou de réparation de tissus cutanés, sous-cutanés ou des cordes vocales, par injection ou implantation chirurgicale directe de fibroblastes cultivés autologues provenant d'un tissu conjonctif, ou dermique, ou aponévrotique, ou de fibroblastes provenant de tissus du chorion de l'intestin ou d'adipocytes. Les fibroblastes cultivés utilisés pour l'augmentation et/ou la réparation d'anomalies cutanées proviennent d'un tissu conjonctif, et/ou dermique et/ou aponévrotique. L'invention concerne en outre une méthodologie qui permet de cultiver des cellules sensiblement débarrassées des protéines potentiellement immunogènes contenues dans le sérum en faisant passer, au stade final de leur culture, les fibroblastes issus de tissus du chorion de l'intestin ou d'adipocytes dans un milieu exempt de sérum, ou encore dans le propre sérum du patient.
Gerigene Medical Corporation
Smart & Biggar
LandOfFree
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