C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
167/37, 167/45,
C12P 21/08 (2006.01) C07K 16/42 (2006.01) C12N 5/12 (2006.01) G01N 33/543 (2006.01) G01N 33/567 (2006.01) G01N 33/577 (2006.01)
Patent
CA 1330961
AUTO-ANTI-IDIOTYPIC MONOCLONAL ANTIBODIES TO RECEPTORS AND USES THEREOF Abstract of the Disclosure A competitive immunoassay for a steroid receptor has been developed in which monoclonal antibodies capable of binding to the steroid receptor are competitively bound by anti-steroid antibodies capable of binding to the steroid. The presence or amount of monoclonal antibody-anti-steroid antibody complex formed is related to the amount of steroid receptor present in the assayed material. A histochemical assay is also provided for detecting the amount of a steroid-receptor complex in a biological sample. This assay involved (1) adding an amount of steroid to the sample to form a steroid-receptor complex; (2) contacting the complex with a monoclonal antibody capable of binding to the complex; (3) removing any unbound monoclonal antibody; (4) adding a detectably labeled antibody capable of binding to the monoclonal antibody; (5) determining the amount of labeled antibody bound to the monoclonal antibody; and (6) determining the amount of steroid-receptor complex. The invention further provides a method of purifying receptors to a steroid hormone using monoclonal antibodies capable of binding to the receptor, and a method for producing a monoclonal anti-idiotypic antibody capable of binding to a steroid receptor.
517784
Cleveland William L.
Edelman Isidore S.
Erlanger Bernard F.
Tzilianos Eftihia
Gowling Lafleur Henderson Llp
The Trustees Of Columbia University In The City Of New York
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