Autoclavable annular chromatograph

G - Physics – 01 – N

Patent

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Details

G01N 30/58 (2006.01)

Patent

CA 2397666

The invention relates to a CAC device comprising a stationary column head, a stationary eluate collecting ring, a rotating body which is located between said column head and said collecting ring and which can rotate about a vertical axis and contains a chromatographic separating medium; and a drive device. Said rotating body (1) is connected to a rotating platform (4) at its bottom end. This rotating platform (4) is slidingly, sealingly guided on the eluate collecting ring (5) and has an extension on the underside. The extension can be coupled to a drive shaft (2) of the drive device (8) with a rapidly detachable connection and the stationary column head, the rotating body (1) with the rotating platform (4) and the attached eluate collecting ring (5) can be connected to form a CAC module and can be removed from the drive shaft (2) as a unit. The invention also relates to an inverse CAC method using the inventive device. According to this method, the sample material is distributed over the entire periphery of the separating medium.

L'invention concerne un dispositif de chromatographie annulaire continue (CAC) comportant une tête de colonne, une bague stationnaire servant à collecter l'éluat, un corps de révolution qui peut tourner autour d'un axe vertical et qui contient la substance de séparation chromatographique, ainsi qu'un organe d'entraînement. Le corps de révolution (1) est raccordé au niveau de son extrémité inférieure à un plateau rotatif (4) qui est guidé de manière coulissante et étanche sur la bague collectrice d'éluat (5). Le plateau rotatif (4) présente, sur son côté inférieur, une partie saillante qui peut être accouplée à un arbre d'entraînement (2) de l'organe d'entraînement (8) par l'intermédiaire d'une liaison pouvant être libérée rapidement. La tête de colonne stationnaire, le corps de révolution (1) comportant le plateau rotatif (4), ainsi que la bague collectrice d'éluat (5) adjacente sont assemblés de manière à former un module CAC et peuvent être retirés individuellement de l'arbre d'entraînement (2). L'invention concerne en outre un procédé inverse de CAC faisant appel au dispositif selon l'invention, le matériau de l'échantillon étant réparti sur l'ensemble de la substance de séparation.

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